N-amino-17alpha-aza-d-homoandrost-5-en-3beta-ol and hydrazones thereof



United States Patent Ofiice 3,374,233 Patented Mar. 19, 1968 wherein Am represents either the amino radical, NH or a substituted amino radical of the formula in which Z represents an alkylidene or cycloalkylidene radical optionally substituted by an aryl or heterocyclic grouping.

Among the unsubstituted alkylidene and cycloalkylidene radicals represented by Z, especially ethylidene, propylidene, isopropylidene, butylidene, sec-butylidene, cyclopentylidene, cyclohexylidene, and homologous bivalent groupings derived from alkanes and cycloalkanes by removal of 2 hydrogen atoms from the same carbon atom are preferred. Likewise preferred embodiments of Z are substituted alkylidene radicals such as the aralkylidene groupings benzylidene halobenzylidene (o, m, or p), methylenedioxybenzylidene (2,3 or 3,4) napthylmethylene (1 or 2), and dihydronaphthylidene (1 or 2), as also the pyridylmethylene (2, 3,-or 4) groupings.

Equivalent to the foregoing compounds for the purposes of this invention are corresponding non-toxic acid addition salts of the formula Am CH3 HO M wherein Am has the meaning assigned above; X represents 1 equivalent of an anion-for example, chloride, bromide, iodide, nitrate, phosphate, sulfate, sulfamate, methyl sulfate, ethyl sulfate, benzenesulfonate, toluenesulfonate, acetate, lactate, succinate, malate, maleate, tartrate, citrate, gluconate, ascorbate, benzoate, cinnamate, or the likewhich, in combination with the cationic portion of a salt aforesaid, is neither pharrnacolo'gically nor otherwise undesirable in physiological dosage; and n represents a positive integer less than 3.

, The compounds to which this invention relates (including the aforesaid acid addition salts) are characterized by valuable pharmacological properties. Thus, for example, they are anti-convulsants, and they also manifest antibiotic activity versus bacteria such as Diplococcus pneumoniae, protoza such as Tetrahymena ge lleii, algae such as Chlorella vulgarz's, and dicotyledenous seed germination.

Preparation of the compounds hereof proceeds by heating l7a-aZa-D-homoandrost-5-en-3fi-ol with sodium nitrite in aqueous ethanol under acid conditions to give the N-nitroso compound, which is reduced to N-amino-17aaza-D-homoandrost-5-en-3B-ol with lithium aluminum hydride in boiling tetrahydrofuran. The amine is converted to the corresponding hydrazones by heating with an appropriate carbonyl compound (Z being defined as before), using 2-propanol as solvent and suffic-ient hydrochloric acid to render the reaction mixture just acid to litmus. The acid addition salts hereof derive from the bases on admixture in 2-propanol solution with l or 2 equivalents of any of various inorganic and strong organic acids wherein the anionic components conform to X as hereinabove set forth.

The following examples describe in detail compounds illustrative of the present invention and methods which have been devised for theirpreparation. However, the invention is not to be construed as limited thereby, either in spirit or in scope, since it will be apparent to those skilled in the art of organic synthesis that many modifications, both of materials and of methods, may be practiced without departing from the purpose and intent of this disclosure. Throughout the examples hereinafter set forth, temperatures are given in degrees centigrade, pressures in millimeters of mercury, and relative amounts of materials in parts by weight, except as otherwise noted.

Example 1 (A) N-nitroso 17a aza D homOandrost-S-en- 3,6-al.To a solution of 24 parts of 17a-aza-D-homoandr0st-5-en-3B-ol in a mixture of 240 parts of ethanol, parts of water, and just suflicient hydrochloric acid to render the solution acid to litmus is added, during 1 hour with stirring at 70", a solution of 12 parts of sodium nitrite in 24 parts of water and sufiicient hydrochloric acid to maintain the acidity level. Stirring at 70 is continued for 1 hour after the addition is complete, whereupon the reaction mixture is cooled to 0 and then filtered to isolate the crystalline precipitate thrown down. The precipitate is taken up in chloroform; and the chloroform solution is consecutively washed with 5% hydrochloric acid, dried over calcium sulfate, and stripped of solvent by vacuum distillation. The residue, recrystallized from 50% aqueous ethanol, affords N- nitroso 17a-aza D homoandrost 5-en-3/3-o1 softening at approximately 182 and melting at 192-194".

(B) N-amino 17a aza D homoan drost S-en- 3fl-0 l.To a suspension of 20 parts of lithium aluminum hydride in 1500 parts of tetrahydrofuran is added, with vigorous agitation during 15 minutes, a solution of 20 parts of N-nitroso 17a aza D homoandrost-S-en- 35-01 in parts of tetrahydrofuran. The resultant mixture is heated at the boiling point under reflux with continued agitation for 16 hours, whereupon 21 parts of Water, 19 parts of aqueous 20% sodium hydroxide, and 54 parts of water are consecutively mixed in. The mixture thus obtained is heated to the boiling point, whereupon r insoluble solids are separated by filtration. The solids are washed with 200 parts of boiling tetrahydrofuran, and the wash liquor is combined with the hot filtrate. From the combined solutions, on cooling, there precipitates N- amino 17a aza D homoandrost 5-en-3fi-ol which, filtered off and dried in air, melts at 249-25 3 (C) N-amino 17a aza D homoandrost 5-en -3;8- ol hydr0chloride.To a suspension of 24 parts of N- amino 17a aza D homoandrost S-en-SB-ol in 280 parts of absolute ethanol at the boiling point under reflux is added just sufficient 2-propanolic hydrogen chloride to acidity the suspension and bring about solution. The solution is cooled and then diluted with 180 parts of anhydrous ether. The solution thus obtained is chilled to The precipitate which forms is filtered off and recrystallized from a mixture of ethanol and ether to give N-amino 17a aza D homoandrost -en-3fl-ol hydrochloride melting at approximately 287 (with browning and gas evolution).

(D) N-ethylideneamino 17a aza D homoan'drost- 5-en-3/3-0l.-To a mixture of 50 parts of N-amino-17aaza D homoandrost-5-en-3B-ol and 1600 parts of 2- propanol at the boiling point under reflux is added just suflicient 2-propanolic hydrogen chloride to render the mixture acid to litmus and effect solution. The solution is cooled, whereupon 8 parts of .acetaldehyde is introduced and the resultant mixture heated at the boiling point under reflux for 3 hours. Solvent is then stripped by vacuum distillation and the residue partitioned between chloroform and Water. The chloroform phase is separated, consecutively washed with water and aqueous 5% potassium carbonate, dried over anhydrous potassium carbonate, and stripped of solvent by vacuum distillation. The resultant residue, recrystallized from aqueous ethanol, affords N- ethylideneamino 17a aza D homoandrost-5-en-3fi-ol sintering at approximately 154 and melting at 159-162". The product has the formula N=OHCH3 C Ha I Example 2 N-isopropylideneamino 17a aza D homoana'rost- 5-en-3B-0L-To a mixture of 50 parts of N-amino-l7aaza-D-homoandrost 5 en-3fi-ol and 1600 parts of 2- propanol at the boiling point under reflux is added just sufficient 2-propanolic hydrogen chloride to render the mixture acid to litmus and effect solution. The solution is cooled, whereupon 100 parts of acetone is introduced and the resultant mixture heated at the boiling point under reflux for 3 hours. The solvent is then stripped by vacuum distillation and the residue partitioned between chloroform and water. The chloroform phase is separated, consecutively washed with water and aqueous 5% potassium carbonate, dried over anhydrous potassium carbonate, and stripped of solvent by vacuum distillation. The residue is N-isopropylideneamino 17a-aza-D-homoandrost- S-en-3fi-ol, having the formula Example 3 N-cyclopentylideneamino 17a aza D-homoandrost- 5-en-3B-0L-Substituti0n of 15 parts of cyclopentanone for the acetone called for in Example 2 affords, by the procedure there detailed, N-cyclopentylideneamino 17aaza-D-homoandrost-S-en-35-01 having the formula 51 CH3 r H o-Q Example 4 N-cyclohexylideneamino 17a -aza D homoandrost- 5-en-3fi-0l.Substitution of 18 parts of cyclohexanone for the acetaldehyde called for in Example 1D affords, by the procedure there detailed, N-cyclohexylideneamino- 17a-aza-D-homoandrost-5-en-35-01 melting at 143-147".

Example 5 N benzylideneamino 17a aza D homoandrost- 5-en-3p-0l.Substitution of 19 parts of benzaldehyde for the acetone called for in Example 2 affords, by the procedure there detailed, N benzylideneamino-17a-aza-D- homoandrost-S-en-B/B-ol, having the formula Example 6 N-(o-chlorobenzylideneamino) 17a aza D homoandrost-S-en-fifi-0l.Substitution of 25 parts of o-chlorobenzaldehyde for the acetone called for in Example 2 affords, by the procedure there detailed, N-(o-chlorobenzylideneamino) 17a aza D homoandrost-S-en- 35-01. The product has the formula Example 7 proximately 225 andmelts at 230 231". The product has the formula Example 8 N-(34-methylenedioxybenzylideneamino) 17a aza- D-hm0andr0st-5-en-3fi-0l.-Substitution of 27 parts of 3,4-methylenedioxybenzaldehyde for the acetaldehyde called for in Example 1D affords, by the procedure there detailed, N (3,4 methylenedioxybenzylideneamino)- 17a aza D homoandrost-S-en-SB-ol which, heated at 150 under 0.1 mm. pressure for 30 minutes, melts at 183-186. The product has the formula Example 9 N (Naphthylmethylene) 17a aza D homvandr0st-5-en-3 -ol. Substitution of 28 parts of l-naphthaldehyde for the acetaldehyde caled for in Example 1D affords, by the procedure there detailed, N-(naphthylmethylene) 17a aza D homoandrost 5 en 3pol melting at 208-210. The product has the formula N=CH- CH9 Example 10 N (3,4-dihydr0-2(1H) naphlhylideneamino) 17aaza-D-h0m0andr0St-5-en-3/3-0l.S11bStitutiOn of 21 parts of Z-tetralone for the acetaldehyde called for in Example 1D affords, by the procedure there detailed, N-(3,4-dihydro-2(1H)-naphthylideneamino)-17a aza D homoandrost-5-en-3fi-ol. The product has the formula Example 11 N (Z-pyridylmethyleneamino) 17a aza D homoandr0st-5-en-3 8-0l.SubStitutiOn of 19 parts of '2- pyridinecarboxaldehyde for the acetaldehyde called for 6 in Example 115 alTords, by the procedure there detailed, N (2 pyridylmethyleneamino) 17a aza D homolandrost-S-en-B fl-ol which, heated at under 0.1 mm. pressure for 2 hours, sinters at approximately 205 and melts at 216-220. The product has the formula Example 12 N (3-pyridylmethyleneamino) 17a aza D homoandr0st-5-en-3fl-ol.Substitution of 19 parts of 3-pyridinecarboxaldehyde for the acetaldehyde called for in Example 1D affords, by the procedure there detailed, N- (3-pyridylmethyleneamino) 17a aza D homoandrost-5-en-3B-ol sintering at approximately 225 and melting at 233237. The product has the formula A} HO Example 13 N (4-pyridylmethyleneamino) 17a aza D homoandrost-5-en-3 8-0L4ubstitution of 19 parts of 4-pyridinecarboxaldehyde for the acetaldehyde called for in Example 1D affords, by the procedure there detailed, N- (4-pyridylmethyleneamino) 17a aza D homoandrost-5-en-3B-ol sinteringat approximately 253 and melting at 261-263". The product has the formula What is claimed is: 1. A compound of the formula N=R CH3 I J Hall;

3. N cyclohexylideneamino-17a-aza-D-homoandrost- S-en-Sfl-ol.

4. N benzylideneamino 17a -aza D homoan- 'drost-5-en-3fi-ol'.

5. N (o-chlorobenzylideneamino) 17a aza D- homoandrost-S-en-BB-ol. s

6. N (4 pyridylmethyleneamino)-17a-aza-D-homoandrost-5-en-3fi-ol.

7. N amino 17a aza-D-homoandrost-5-en-3fl-ol hydrochloride.

8 References Cited UNITED STATES PATENTS 2,738,350 3/1956 Mazur 260289 3,158,609 11/1964 Hamilton et'al 260240 10 JOHN D. RANDOLPH, Primary Examiner. 

1. A COMPOUND OF THE FORMULA
 4. N - BENZYLIDENEAMINO - 17A - AZA - D - HOMOANDROST-5-EN-3B-OL. 